The present investigation was carried out to study the prevalence of leptospirosis in dairy cows with reproductive disorders in and around Kamrup district of Assam. A total of 130 sera collected from dairy cows with reproductive disorders in different localities of Assam were screened for leptospirosis by MAT. The seroprevalence of leptospirosis in dairy cows with reproductive disorders was found to be 14.62 per cent. Twelve Leptospira antigen serovars: L. Australis, L. Autumnalis, L. Ballum, L. Bataviae, L. Canicola, L. Grippotyphosa, L. Hebdomedis, L. Pomona, L. Pyrogenes, L. Sejroe, L. Icterohaemorrhagaie, and L. Javanica were used, to screen for leptospirosis. Leptospira antibodies were detected in four serovars: Australis, Autumnalis, Ballum and Bataviae. Australis was found to be the most circulating serovar with 47.37 per cent. The reproductive disorders reported in the present study were: repeat breeding 41 (31.54%), abortion 34 (26.15%), endometritis 25 (19.23%), post-partum anoestrus 14 (10.77%), dystocia 5 (3.85%), retention of foetal membrane 4 (3.07%), anovulation 2 (1.53%), uterine prolapse 2 (1.53%), silent oestrus 2 (1.53%) and cystic ovaries 1 (0.77%). Repeat breeding was found to be the most prevalent reproductive disorder (31.54%) followed by cases of abortion (26.15%) in different localities of Assam.