Urinary tract infections (UTI) are one of the most common infections causing morbidity in community and health care setup. Women in reproductive age group (15-49 yrs) are more prone to UTI. This study was undertaken to determine the prevalence of UTI, the microbial etiology and the antibiotic susceptibility profile of the isolates from women with symptomatic UTI in a tertiary care centre in north India. Clean catch midstream urine were processed by semi-quantitative culture and isolates identified by standard biochemical tests. Antibiotic sensitivity was performed by Kirby-Bauer disc diffusion test and data analyzed from January 2013 to September 2015. A total of 3141 cases were studied. Prevalence of UTI varied from 16.69% in 2013, 9.77% in 2014 to 8.45% in 2015. Majority of UTI occurred in the age group 21-30 yrs. E.coli was the commonest isolate causing UTI followed by E.faecalis. E.coli showed considerable resistance to ampicillin and fluoroquinolones but performed better against nitrofurantoin with greater than 90% susceptibility. Constant survey of uropathogens and their susceptibility profile is essential for deciding on the empirical treatment of UTI.