Acute kidney injury (AKI) refers to a clinical syndrome characterized by rapid loss of renal function, which may further aggrevates into chronic kidney damage (CKD) or even end-stage renal disease (ESRD). Recently, the term ARF (Acute Renal Failure) has been replaced by Acute Kidney Injury. Cisplatin is a platinum containing drug widely used as chemotherapeutic agent with dose-limited nephrotoxicity. The present study envisaged the evaluation of therapeutic potential of N-acetylcysteine (NAC) in wistar rats against acute kidney injury induced by cisplatin. This study comprises of three groups: Group I: Heathy control group, Group II: Positive control group (Cisplatin only), Group III: NAC treatment group (Cisplatin+N-Acetylcysteine group). Oxidative stress indices like glutathione (GSH) and Malondialdehyde (MDA) were estimated in tissue homogenate sample with the help of commercial available. Renal injury was assessed via estimation of serum creatinine and urea level. Kidney tissues were collected for histopathological evaluation at the end of the study on day 28th. The mean value of GSH was significantly (P< 0.05) higher in Group III in comparision to Group II in kidney tissue homogenate. MDA value was significantly (P< 0.05) higher in Group II and lower in Group III and Group I. BUN and Creatinine levels were significantly higher in Group II as compared to other two groups. Group II rats were showing severe histopatholohical changes in kidney tissue as compared to group III. Therefore, this study can conclude that NAC can alleviate AKI induced by Cisplatin and has a good therapeutic potential against cisplatin nephroprotoxicity.