A detailed investigation was carried out on the 16 naturally occurring cases of canine babesiosis from Jaipur (Rajasthan). The aim of this study was to determine the Clinico-haemato-biochemical changes in dogs naturally infected with Babesia. The present study reports the significant changes in hematology and biochemistry i.g. Hemoglobin (9.37 ± 0.57 g/dl), Total erythrocyte count (6.24 ± 0.42 × 106 /µL), Platelets count (1.49 ± 0.17 × 105 /µL), ALT (84.66 ± 15.15 U/L), BUN (40.12 ± 9.59 mg/dL), and Serum Creatinine (2.74 ± 0.68 mg/dL) in affected dogs when compared to healthy dogs. Clinical and Physiological observations were indicative of severity of babesiosis in dogs. This disease is multisystemic and complex.