Ocular ultrasonography is an important diagnostic tool which permits precise assessment of the eyeball and orbital structures contributing to a quick diagnosis and better therapy. The objective of the study was to describe the ultrasonographic appearance of the normal poultry eyes and to compare the biometric measurements of both the eyes. Hundred eyes from 50 poultry birds were assessed after a routine ophthalmic examination using an 10 MHZ linear transducer. Ultrasound examination could clearly differentiate different parts of the internal eye, including the pecten oculi in the vitreous. Ocular biometry was performed by B-scan of the following structures (in mm): Corneal thickness (CT) (RE: 0.13 ± 0.07, LE: 0.12 ± 0.07), Axial length (AL) (RE: 1.13 ± 0.01, LE: 1.05 ± 0.18), Lens width (LW) (RE: 0.33 ± 0.15, LE: 0.34 ± 0.19). Lens length (LL) (RE: 0.71 ± 0.02, LE: 0.65 ± 0.02). There was a uniform correlation between the ocular structures, however, no statistical difference was observed in the parameters of both the eyes. The anatomic and biometric assessment will be helpful in identifying routine clinical abnormalities.