Year: 2019 | Month: June | Volume 8 | Issue 1
Antibiotic Sensitivity Pattern and Safety Evaluation of Isolated Strains of Fecal Origin L. reuteri
Abstract:
Forty isolates of L. reuteri from human infant fecal sample were previously isolated and identified in Microbial Metabolite laboratory of Dairy Microbiology Division, National Dairy Research Institute, Karnal. An initial attempt in context to study safety of 40 strains of L. reuteri was done in terms of decarboxylation of amino acids i.e. their ability to produce biogenic amines i.e. tyramine, histamine and puteriscine,antibiotic susceptibility. Physiological investigation of all the 40 L. reuteri strains for the presence of virulence characters revealed that incidence of such traits was not present as none of the isolate was found to possess β-hemolytic activity, DNAse activity and/or gelatinase activity. All 40 Lactobacillus reuteri strains from infant feces were subjected to antibiotic susceptibility testing using the agar diffusion method. All of them were sensitive to erythromycin (except LR1), rifampicin (except LR25), of loxocin (except LR15 and LR20), novobiocin (except LR1, LR6, LR15, LR21, LR22, LR23, LR25, LR26, LR34, LR 36 and LR38), bacitracin, chloramphenicol and clindamycin (except LR1 and LR25), but they were resistant to polymixin B (except LR15), gentamycin (except LR21), cefazolin, ampicillin (except LR15, LR20 and LR21), kanamycin, amikacin, vancomycin, cephalothin, cefuroxime (except LR5, LR9, LR34, LR38 and LR39).All the strains were found to be safe for human use.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
